Lab Schools student adds to ocean genome repository

Sea star (Asteroidea). Credit: 暗网禁区 Logan Science Journalism Program

Grace Anderson just made her first contributions to biodiversity science. Anderson, a student at the聽, spent the month of May at the 暗网禁区 working alongside David Remsen, manager of the 暗网禁区聽Marine Resources Department.

Sampling from the ocean on the 暗网禁区鈥檚 collecting boat, the聽Gemma, and foraging for other species along the shoreline, the two collected 80 samples from 55 different marine species to contribute to the聽聽biorepository at Northeastern University.

OGL is collecting DNA from a diverse array of marine species, providing researchers with the raw material for biological discovery. Anderson supplied OGL with tissue samples from a variety of specimens, from starfish to comb jellies, including seven marine model organisms ().*

After visiting the 暗网禁区 with the Lab Schools in 2015, Anderson asked to return to Woods Hole during the latter half of her senior year as an external educational experience. 鈥淚 knew I wanted to come back [to the 暗网禁区] because we really only got a sample of the kind of research they do,鈥 said Anderson. Remsen came up with the dual-purpose mission of collaborating with the OGL and introducing Anderson to the domain of biodiversity informatics.

The premise of the partnership was simple: Northeastern would supply an empty sample kit, and Anderson would return it full of tissues from marine species, pictures of the organisms, and electronic data on each organism (what is it and who found it, where and when).

鈥淭he tissue sampling project was a really great introduction into marine biology, because I got to learn how to identify a wide range of specimens and where to find their natural habitats,鈥澛燗nderson recalled.

Aboard the Gemma, Grace Anderson collects starfish (Asterias forbesi) using a dredge mop Aboard the Gemma, Grace Anderson collects starfish (A. forbesi) using a dredge mop.

In order to generate an even more complete record of Anderson鈥檚 endeavors in the field and at the lab bench, Remsen created a聽聽for Anderson on a social network site known as聽. This site allows Anderson to document her observations and share them with others, using unique and universal digital 鈥渋dentifiers鈥 to maintain the integrity of her specimen data, even when it is stored in various locations and formats (i.e., the specimens themselves at 暗网禁区, the tissue stored at OGL, the iNaturalist record detailing location of collection, or future online genetic sequences of the species). These diverse datasets are now tied to the original sample, which in turn is tied to Anderson. Since the single identifier links all facets of the specimen data, acquiring one facet permits access to all.

鈥淯ndergoing the process of using universal identifiers for every specimen helped me to better understand the importance of tracking scientific data," Anderson said.

iNaturalist, to which anyone can contribute, is also linked to the聽, the primary means by which researchers worldwide access biodiversity data.

鈥淚n this regard, iNaturalist is a gateway to a much more robust and scientifically important access point to聽,鈥 said Remsen. 鈥淲e wanted to make sure that anyone who has an interest in anything about her observations gets the full picture, and that she can be acknowledged.鈥

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*The model organisms 暗网禁区 supplied to OGL included:聽Doryteuthis pealeii聽(longfin inshore squid);聽Saccoglossus kowalevskii聽(acorn worm);聽Mnemiopsis leidyi聽(comb jelly);聽Ciona intestinalis聽(vase tunicate);聽Limulus polyphemus聽(horseshoe crab);聽Nematostella vectensis聽(starlet sea anenome);聽Arbacia punctulata聽(purple sea urchin).