DAY 1-Tuesday, August 13, 2024
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5:00 PM-6:00 PM
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Swope
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Walk around Woods Hole (optional; meet in front of Swope)
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Course Staff
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6:00 PM-7:00 PM
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Speck
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Welcome and orientation
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Alison North, Joerg Bewersdorf
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7:00 PM-8:00 PM
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Swope Terrace
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Pizza Party
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8:00 PM-9:00 PM
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Speck
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Lecture: Grand overview of the course
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Alison North
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DAY 2 - Wednesday, August 14, 2024
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8:15 AM-9:30 AM
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Speck
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Lecture: Foundations of microscopy 1 - geometrical optics
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Joerg Bewersdorf
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9:30 AM-10:00 AM
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Break
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10:00 AM-11:00 AM
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Speck
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Lecture: Foundations of microscopy 2 - lenses and optical systems
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Joerg Bewersdorf
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11:00 AM-12:00 PM
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Loeb 2nd floor
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Lab: Basic Sample preparation
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Course Staff
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12:00 PM-1:00 PM
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Swope
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Lunch
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1:00 PM-3:15 PM
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Loeb 2nd floor
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Lab: Build basic microscopes
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Course Staff
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3:15 PM-3:30 PM
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Break
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3:30 PM-6:30 PM
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Loeb 2nd floor
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Lab: Practice with commercial microscope stands
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Course Staff
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6:30 PM-7:30 PM
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Swope
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Dinner (meeting for vendors: last 15 min of dinner)
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7:30 PM-9:00 PM
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Speck
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Lecture: Foundations of microscopy 3 - wave optics
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Joerg Bewersdorf
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9:00 PM-10:00 PM
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Loeb 2nd floor
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Demonstration: Abbe diffraction apparatus
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Chris Bjornsson
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DAY 3 - Thursday, August 15, 2024
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8:15 AM-9:30 AM
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Speck
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Lecture: Transmitted light 1
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Sharon King
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9:30 AM-9:45 AM
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Break
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9:45 AM-11:15 AM
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Speck
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Lecture: Cameras
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Stephanie Fullerton
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11:15 AM-12:15 PM
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Loeb 2nd floor
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Lab: Phase contrast microscopy
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Course Staff
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12:15 PM-1:15 PM
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Swope
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Lunch
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1:15 PM-3:15 PM
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Loeb 2nd floor
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Lab: ImageJ basics
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Andrew McCall
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3:15 PM-4:15 PM
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Loeb 2nd floor
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Lab: Use of cameras
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Andrew McCall and Course Staff
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4:15 PM-5:30 PM
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Speck
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Lecture: Transmitted light 2
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Sharon King
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5:30 PM-6:30 PM
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Loeb 2nd floor
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Lab: DIC and polarization (part 1)
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Sharon King and Course Staff
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6:30 PM-7:30 PM
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Swope
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Dinner (meeting for vendors: last 15 min of dinner)
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7:30 PM - 10:30 PM
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Loeb 2nd floor
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Lab: DIC and polarization (part 2)
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Sharon King and Course Staff
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DAY 4 - Friday, August 16, 2024
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8:15 AM-9:00 AM
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Speck
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Discussion of lab exercises
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Sharon King
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9:00 AM-9:45 AM
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Speck
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Lecture: Photophysics of fluorescence and dye chemistry
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Luke Lavis
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9:45 AM-10:45 AM
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Speck
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Lecture: Introduction to the fluorescence microscope
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Alison North
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10:45 AM-11:00 AM
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Break
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11:00 AM-12:30 PM
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Loeb 2nd floor
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Lab: Fluorescence microscopy (part 1)
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Course Staff
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12:30 PM-1:30 PM
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Swope
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Lunch
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1:30 PM-2:00 PM
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Speck
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Lecture: Chemical tags for functional imaging
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Luke Lavis
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2:00 PM-3:00 PM
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Speck
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Lecture: Sample preparation techniques
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Alison North
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3:00 PM-3:30 PM
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Break
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3:30 PM-4:00 PM
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Loeb 2nd Floor
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Lab: Cleaning optical filters
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Mark Lessard
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4:00 PM-6:00 PM
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Loeb 2nd Floor
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Lab: Fluorescence microscopy (part 2)
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Course Staff
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6:00 PM-7:00 PM
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Swope
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Dinner (meeting for vendors: last 15 min of dinner)
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7:00 PM-9:30 PM
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Loeb 2nd floor
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Lab: Build widefield fluorescence microscope
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Course Staff
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9:30 PM-11:00 PM
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Loeb 2nd floor
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Commercial faculty appreciation event
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DAY 5 - Saturday, August 17, 2024
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8:15 AM-8:30 AM
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Speck
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Discussion of lab exercises
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Alison North, Joerg Bewersdorf
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8:30 AM-9:30 AM
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Speck
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Lecture: Fluorescent proteins
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Alison Tebo
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9:30 AM-10:00 AM
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Break
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10:00 AM-11:00 AM
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Speck
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Lecture: Imaging dynamics (incl. FRET, FLIM, FCS)
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Mathew Tantama
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11:00 AM-12:00 PM
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Speck
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Lecture: Biosensors
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Alison Tebo
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12:00 PM-1:00 PM
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Swope
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Lunch
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1:00 PM-3:15 PM
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Loeb 2nd floor
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Lab: FRET & FLIM
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Alison Tebo, Mathew Tantama, Course Staff
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3:15 PM-4:00 PM
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Discussion of FRET & FLIM lab exercises
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Alison Tebo, Mathew Tantama, Course Staff
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4:00 PM-5:00 PM
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Speck
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Lecture: Tissue Clearing
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Doug Richardson
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5:00 PM-5:15 PM
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Break
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5:15 PM-6:00 PM
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Speck
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Lecture: Principles of digital imaging 1
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Patrick La Riviere
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6:00ʲ-7:00ʲ
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Swope
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Dinner (meeting for vendors: last 15 min of dinner)
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7:00ʲ-8:00ʲ
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Speck
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Lecture: Principles of digital imaging 2
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Patrick La Riviere
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8:00 PM - 9:30 PM
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Speck
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Lab: Digital imaging
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Patrick La Riviere and Course Staff
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DAY 6 - Sunday, August 18, 2024
FALMOUTH ROAD RACE - EXPECT ROAD CLOSURES!
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8:15 AM-9:00 AM
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Speck
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Discussion of lab exercises
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Patrick La Riviere, Joerg Bewersdorf
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9:00 AM-9:45 AM
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Speck
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Lecture: Evaluating optical performance using PSFs
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Lynne Turnbull
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9:45 AM-10:00 AM
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Break
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10:00 AM-12:00 PM
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Loeb 2nd floor
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Lab: Resolution measurements
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Joerg Bewersdorf
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12:00 PM-12:30 PM
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Loeb courtyard
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Group Photo (followed by meeting for vendors)
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Mark Lessard
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12:30 PM-1:00 PM
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Lunch
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1:00 PM-5:00 PM
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Afternoon off
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5:00 PM-6:00 PM
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Speck
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Lecture: Deconvolution
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Sid Shaw
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6:00 PM-7:00 PM
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Swope
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Dinner (NO vendor meeting)
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7:00 PM-8:30 PM
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Speck
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Lecture: Confocal microscopy
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Sid Shaw
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8:30 PM-8:45 PM
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Break
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8:45 PM-9:45 PM
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Speck
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Discussion of lab exercises
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Sid Shaw
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DAY 7 - Monday, August 19, 2024
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8:15 AM-9:15 AM
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Speck
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Lecture: Multiphoton microscopy
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Cristina Rodriguez
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9:15 AM-12:15 PM
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Loeb 2nd floor
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Lab: Deconvolution, confocal, multiphoton (part 1)
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Sid Shaw, Doug Richardson, Cristina Rodriguez and Course Staff
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12:15 PM-1:15 PM
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Swope
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Lunch
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1:15 PM-4:15 PM
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Loeb 2nd floor
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Lab: Deconvolution, confocal, multiphoton (part 2)
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Sid Shaw, Doug Richardson, Cristina Rodriguez and Course Staff
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4:15 PM-4:45 PM
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Break
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4:45 PM-5:15 PM
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Speck
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Lecture: ImageJ tutorial on quantitative analysis
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Sid Shaw
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5:15 PM-6:15 PM
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Speck
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Lab: Quantification & analysis (part 1)
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Sid Shaw
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6:15 PM-7:15 PM
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Swope
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Dinner (meeting for vendors: last 15 min of dinner)
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7:15 PM-8:45 PM
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Speck
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Lab: Quantification & analysis (part 2)
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Sid Shaw
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8:45 PM-10:00 PM
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Speck
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Discussion of Lab Exercises
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Sid Shaw
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DAY 8 - Tuesday, August 20, 2024
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8:30 AM-9:00 AM
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Speck
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Lecture: Instrumentation for live-cell imaging
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Michelle Itano
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9:00 AM-10:00 AM
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Speck
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Lecture: Imaging live specimens without killing them
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Binyam Mogessie
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10:00 AM-10:15 AM
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Break
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10:15-11:15 AM
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Speck
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Lecture: Light sheet microscopy
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Wes Legant
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11:15-11:30 AM
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Break
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11:30 AM-12:30 PM
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Speck
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Lecture: Adaptive optics
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Cristina Rodriguez
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12:30 PM-1:30 PM
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Swope
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Lunch
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1:30-2:30 PM
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Speck
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Lecture: Super-resolution 1 - SIM methods
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Alison North, Joerg Bewersdorf
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2:30-5:30 PM
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Loeb 2nd Floor
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Lab: Light-sheet microscopes, SIM, iSIM (part 1)
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Course Staff
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5:30-6:00 PM
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Speck
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Lecture: Super-resolution 2 - Expansion Microscopy
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Joerg Bewersdorf
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6:00 PM-7:00 PM
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Swope
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Dinner (meeting for vendors: last 15 min of dinner)
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7:00 PM-9:00 PM
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Loeb 2nd Floor
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Lab: Light-sheet microscopes, SIM, iSIM (part 2)
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Course Staff
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9:00-10:30 PM
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Speck
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Tech Bites
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DAY 9 - Wednesday, August 21, 2024
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8:30 AM-9:30 AM
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Speck
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Lecture: Spatial Omics
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Jacqueline Leung
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9:30-10:15
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Speck
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Lecture: Super-resolution 3 - STED
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Joerg Bewersdorf
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10:15 AM-10:30 AM
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Break
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10:30 AM-11:30 AM
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Speck
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Lecture: Super-resolution 4 - Single-molecule localization techniques
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Joerg Bewersdorf
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11:30 AM-12:15 PM
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Loeb 2nd floor
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Lab: STED & single-molecule localization (part 1)
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Course Staff
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12:15 PM-1:15 PM
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Swope
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Lunch
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1:15 PM-2:00 PM
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Loeb 2nd Floor
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Lab: STED & single-molecule localization (part 2)
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Course Staff
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2:00 PM-2:15 PM
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Swope
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Break
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2:15 PM - 3:00 PM
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Speck
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Lecture: Colocalization
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Alison North
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3:00 PM - 6:00 PM
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Lb 2nd floor
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Lab: Rotations on specialized instruments/demos
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Course Staff
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6:00 - 7:00 PM
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Swope
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Dinner (NO vendor meeting)
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7:00-10:00 PM
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Loeb 2nd Floor
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Lab: Time on instruments/demos (part 1)
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DAY 10 - Thursday, August 22, 2024
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8:30 AM-12:00 PM
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Speck
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Lab: Time on instruments / demos (part 2)
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Course Staff
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12:00 PM-1:00 PM
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Swope
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Lunch
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1:00 PM-2:00 PM
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Speck
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Discussion of super-resolution
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Alison North, Joerg Bewersdorf
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2:00 PM-2:45 PM
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Speck
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Lecture: Ethics and good practices in microscopy
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Michelle Itano
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2:45 PM-3:45 PM
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Speck
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Career Panel
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Alison North, Joerg Bewersdorf
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3:45 PM-4:45 PM
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Final Discussion
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Alison North, Joerg Bewersdorf
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4:45 PM-5:00 PM
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Break
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5:00 PM-6:00 PM
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Speck
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Horizons Lecture
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Florian Jug
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6:00 PM-6:30 PM
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Break
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6:30 PM-9:00 PM
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Swope Terrace
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Banquet
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DAY 11 - Friday, August 23, 2024
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Morning
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Check-out after Breakfast
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