Back to Biology of the Inner Ear: Experimental and Analytical Approaches BIE Protocols Home Educational Opportunities Advanced Research Training Courses Course Offerings Biology of the Inner Ear: Experimental and Analytical Approaches BIE Protocols Many of the protocols used on the BIE course have recently been compiled into a book in the Neuromethods series from Springer Protocols. . In situ hybridization RNAscope (Walters – 2019) Electrophysiology (Ashmore – 2009) Frog Saccule Electrophysiology (Eatock 2011) Immunohistochemistry General Immuno Protocol General Double-label Immuno Protocol BrdU HCl-free, with nuclease protocol EdU Immuno Protocol Math1 Immuno Protocol Myo6 Immuno Protocol Sox2 Immuno Protocol BrdU Protocol (Warchol – 2011) Inner Ear Dissections Dissection of Mouse utricle (Geleoc – 2011) Bullfrog Sacculus Dissection() Chick Cochlea Dissection(ǰɾ) Chick Dissection(ٱ) Chick Utricle Dissection(ǰɾ) Dissection Instruments(ǰɾ) Mouse Dissection (Kelley) Mouse Utricle Dissection(ǰɾ) Mouse Utricle and Organ of Corti(DZ-ұdz) SYLGARD Dishes(ǰɾ) Inner Ear Explant Cultures() Inner Ear Gene Transfection Mouse Cochlea Electroporation (Kelley – 2009) Mouse in utero Electroporation (Brigande – 2009) Inner Ear Paintfills Inner Ear Paintfill (Wu – 2009) (ǰ) SEM sample prep (Walters – 2019)