Wednesday, 4/24/2024
|
1:30 - 2:30 PM
|
Check in and get picture ID
|
3:00 - 4:00
|
Welcome and course orientation
|
Course Directors, Dir. Ed
|
Speck Auditorium
|
4:00 - 4:50
|
LECTURE and LAB: Ray Optics: Making an image and ray tracing
|
Mattheyses
|
Loeb 260 (Main Lab)
|
5:00 - 6:00
|
Wine and Cheese reception
|
Tent outside Swope
|
6:00 - 7:00
|
DINNER
|
7:00 - 7:30
|
LAB Continued
|
Mattheyses
|
Main lab
|
7:30 - 7:50
|
LECTURE: Ray optics: Apertures and field stops
|
Mattheyses
|
Main lab
|
7:50 - 8:30
|
LAB: Group work with irises
|
Mattheyses/ Commercial Faculty
|
Main lab
|
8:30 - 8:45
|
LECTURE: Ray optics: Building a microscope
|
Mattheyses
|
Main lab
|
8:45 - 9:30
|
LAB: Building a microscope condenser in Kohler illumination; bulding the imaging path
|
Mattheyses/ Commercial Faculty
|
Main lab
|
9:30 - 9:50
|
LECTURE: Report out on condenser. Imaging onto a sensor
|
Mattheyses
|
Main lab
|
9:50 - 10:30
|
LAB: Building the imaging path; measuring the magnification of your microscope
|
Mattheyses/ Commercial Faculty
|
Main lab
|
Thursday, 4/25/2024
|
8:45 - 8:50 AM
|
Daily Overview
|
Laevsky
|
Main lab
|
8:50-10:30
|
LECTURE: Wave Optics: Infinity-corrected objectives, Diffraction and Resolution; LAB: Measuring the resolution of your microscope
|
Mattheyses
|
Main lab
|
10:35-11:35
|
What is a digital image? (array of numbers, bit depth, LUTs etc)
|
Maddox
|
Speck
|
11:35 - 12:30
|
Wave optics, diffraction and contrast generation
|
Kner
|
Speck
|
12:30 - 1:30 PM
|
LUNCH (students need to be there by 1)
|
1:30 - 2:30
|
Optics
|
Ross
|
Speck
|
2:30 - 3:30
|
Image Processing Lecture: FIJI
|
Wilson
|
Speck
|
3:40-5:30
|
Image Processing Lab: FIJI
|
Wilson/Directors
|
Main lab
|
5:40 - 6:30
|
Fourier
|
Kner
|
Speck
|
6:30 - 7:30
|
Dinner
|
7:30-8:00
|
Abbe demonstration
|
Main lab
|
8:00 - 10:30
|
lightning talks
|
Mattheyses
|
Main lab
|
Friday, 4/26/2024
|
8:45 - 9:00 AM
|
Daily Overview
|
Laevsky
|
Speck
|
9:00 – 10:00
|
Phase Contrast & Darkfield Lecture
|
Mehta
|
Speck
|
10:00 - 12:30
|
Phase Contrast & Darkfield Laboratory
|
Mehta/Kner/ Commercial Faculty
|
Main lab
|
12:30 - 1:30 PM
|
LUNCH
|
1:30 - 2:30
|
Polarization and DIC
|
Mehta
|
Speck
|
2:30 - 4:30
|
Polarization and DIC Laboratory
|
Mehta/ Kner
|
Main Lab
|
4:30-5:30
|
PSF/trans/fluor
|
Laevsky
|
Speck
|
5:30-6:30
|
DINNER
|
6:30-8:00
|
Fluorescence Lecture
|
Salmon
|
Speck
|
8:00-11:00
|
Fluorescence Lab
|
Salmon
|
Main lab
|
Saturday, 4/27/2024
|
8:45 - 9:00 AM
|
Daily Overview
|
Kner
|
Speck
|
9:00 - 10:30
|
Digital Detectors
|
Watkins
|
Speck
|
10:30-12:00
|
Quant lab
|
Course Directors
|
Main lab
|
12:00-1:00
|
LUNCH
|
1:00-3:00
|
Quant Lab
|
Course Directors/ Commercial Faculty
|
Loeb var
|
3:00-3:30
|
Quant discussion
|
Course Directors/ Commercial Faculty
|
Main lab
|
3:30-5:30
|
Noise Lab
|
Course Directors
|
Main lab
|
5:30-6:30
|
DINNER
|
6:30-8:00
|
Fluorescence Proteins and Probes
|
Palmer
|
Speck
|
8:00-10:00
|
FRET Biosensors Lab
|
Palmer
|
Loeb various
|
10:00-11:00
|
Vendor Talks
|
Main Lab
|
Sunday, 4/28/2024
|
8:45 - 9:00 AM
|
Daily Overview
|
Mattheyses
|
|
9:00 - 10:00
|
Quantifying Cell Mechanics
|
Salaita
|
Speck
|
10:00 -10:30
|
A/O demo
|
Vincent
|
Main Lab
|
10:30 -12:45
|
Live Cell Lecture & Lab
|
Maddox
|
Main Lab
|
12:45-2 PM
|
LUNCH
|
2-5:30
|
Live Cell
|
Maddox/Mattheyses
|
Loeb var
|
5:30-6
|
Discussion
|
Maddox/Mattheyses
|
Loeb var
|
6 - 7:00
|
DINNER
|
7:00 - 8:00
|
3D Microscopy I: The Problems and a Wide-Field Solution
|
Shaw
|
Speck
|
8:00- 11:00
|
Deconvolution Laboratory Exercises
|
Shaw
|
Loeb var
|
Monday, 4/29/2024
|
8:45 – 9:00 AM
|
Daily Overview
|
Mattheyses
|
Speck
|
9:00-10:00
|
3D Microscopy II: Point-scanning Solutions and Their Problems
|
Shaw
|
Speck
|
10:00-12:30
|
Confocal and Multiphoton Laboratory Exercises
|
Shaw
|
Loeb var
|
12:30-1:30 PM
|
LUNCH
|
1:30-2:00
|
3D Microscopy Discussion I
|
Shaw
|
Speck
|
2:00-3:00
|
3D Microscopy III: Analysing Performance and Choosing Methods
|
Shaw
|
Speck
|
3:00-5:30
|
Quantitative Evaluation of Performance in 3D Microscopy
|
Shaw
|
Loeb var
|
5:30-6:30
|
DINNER
|
6:30-7:00
|
3D Microscopy Discussion II
|
Shaw
|
Speck
|
7:00-8:00
|
TIRF Lecture
|
Taraska
|
Speck
|
8:00-11:00
|
TIRF Lab
|
Mattheyses/Taraska
|
Loeb var
|
Tuesday, 4/30/2024
|
8:45 – 9:00 AM
|
Daily Overview
|
Kner
|
Speck
|
9:00 - 10:00
|
SR SMM Localization Lecture
|
Anna-Karin
|
Speck
|
10:00 - 11:00
|
SR SMM Single particle tracking lecture
|
Lidke
|
Speck
|
11:00-12:00
|
SR SIM/STED lecture
|
Hesper Rego
|
Speck
|
12:00-1:00
|
Lunch
|
1:00-5:30
|
SR lab rotations
|
Loeb various
|
5:30-6:30
|
Dinner
|
Main lab
|
6:30-8:30
|
SR lab rotations
|
8:30-9:00
|
SR Discussion
|
Anna-Karin, Diane, Hesper
|
Main Lab
|
9:00 - 10:00
|
Ethics
|
Directors
|
Main lab
|
Wednesday, 5/1/2024
|
8:15 - 8:30 AM
|
Daily Overview
|
Kner
|
Main lab
|
8:30-9:30 AM
|
LSFM Lecture
|
Kumar
|
Speck
|
9:30-12:00
|
LSFM Lab
|
Kumar
|
Loeb various
|
12:00 - 1:00 PM
|
Lunch
|
1:00-3:00 PM
|
LSFM Lab
|
Loeb various
|
3:00-6:00 PM
|
FREE TIME
|
6:00-7:00
|
Dinner
|
7:00 - 8:00
|
Advanced Image Analysis
|
Beth C. / Simon N.
|
Speck
|
8:00 - 10:00
|
Analysis Lab
|
Simon N.
|
Main lab
|
Thursday, 5/2/24
|
8:45 - 9:00 AM
|
Daily Overview
|
Mattheyses
|
Main lab
|
9:00 - 12:00
|
Machine Learning for Image Processing Laboratory
|
Cortes
|
Main lab
|
12:00 - 1:00 PM
|
LUNCH
|
1:00 - 4:00
|
UC2
|
UC2
|
Main lab
|
4:00 - 5:30
|
Computer-hardware communication, open source microscope control
|
UC2
|
Main lab
|
5:30 - 6:00
|
Break
|
6:00 - 7:00
|
Inoue Lecture
|
Margaret A. Titus
|
Meigs Room
|
7:00 - 9:00
|
Clambake
|
everyone!
|
Swope Tent
|
Friday, 5/3/24
|
Depart
|